Vol. 11, Issue 2 (2022)

Author(s):
Shubham D Patil, RS Deshpande, SV Sawardekar, PD Karanje, PS Bhosale and RS Shinge

Abstract:
The present study was carried out to screen thirty three rice genotypes with 19 simple sequence repeat (SSR) markers to identify tolerant rice varieties for biotic and abiotic stresses and to establish rice genotype profiles using SSR markers. DNA was isolated from 33 rice variety and was subjected to molecular profiling by using 19 SSR markers. Genetic profiles were determined by using MVSP software. Amongst the 33 rice genotypes studied, Blast tolerant linked allele was observed in Ratnagiri 1 and Sahyadri 3 genotypes. Bacterial Blight tolerant linked allele was found in Karjat 1, Sahyadri 1, Sahyadri 2 and Sahyadri 4 genotypes. Gall midge tolerant linked alleles were showed in Karjat 2, Ratnagiri 3 and Ratnagiri 7 genotypes. Brown Plant Hopper tolerant linked alleles were present in Ratnagiri 5 genotype. Genotypes Ratnagiri 24 and Ratnagiri 711 were observed tolerant linked allele to drought stress. Salt tolerant linked allele identified in Panvel 1, Panvel 2 and Panvel 3. All 19 primers amplified and showed 100% polymorphism. Total 100 alleles were observed with average of 5.26 alleles per primer. PIC values ranged from 0.27 to 0.894 with an average PIC value of 0.65 per primer. The genetic distance ranged from 0.022 to 0.92 and disclosed wide variability. All 33 rice genotypes divided into two clusters, I cluster containing 19 genotypes and II cluster containing 14 genotypes. This concludes that the molecular screenings of rice varieties using SSR markers provided sufficient knowledge on traits of biotic and abiotic tolerance at molecular level. This study will be used for future strategic breeding program for crop improvement.