Vol. 11, Issue 2 (2022)

Common bean infected with Colletotrichum lindemuthianum negatively effects the growth and development of bean plants. It is hypothesised that pathogen-plant interactions usually induce hypersensitive response by generating H₂0₂ and lipid peroxidation of cell membrane in response to fungal infections. This study was conducted in common bean inoculated with pathogen C. lindemuthianum of two susceptible (BR 104 & BR 50) and one resistant (VL 63) common bean varieties. The pathogen was inoculated using leaf spray method during early flowering stage and plants were observed for changes at 3^rd DAI, 6^th DAI and 9^th DAI. The leaf samples were collected to know the biochemical defense responses generated after inoculation of the pathogen. On 3^rd day, electrolyte leakage percentage was maximally increased in VL 63 and BR 104 inoculated with T1 followed by T2 as compared to control. In contrast, on 6^th day highest percent increase was recorded with T1 treatment in BR 50 whereas least percent increase was recorded in VL 63 inoculated with T2 compared to control. In response to C. lindemuthianum infection in common bean, the H₂O₂ levels significantly increase in resistant variety VL 63 during 3^rd and 6^th days of inoculation however, the susceptible varieties of common bean displayed no significant changes. The level of lipid peroxidation was significantly enhanced on 6^th and 9^th day as compared with uninoculated control plant in BR50 These results suggest that bean infected with C. lindemuthianum displays various biochemical responses an infected with C. lindemuthianum as a defense mechanism with days of disease development.