Vol. 11, Issue 2 (2022)

Author(s):
Rekha Choudhary, Mohan Lal Jakhar, Rakesh Kumar Prajapat, Ravi Kumar, Komal Sekhawat and Swarnlata Kumawat

Abstract:
Leaf, Shoot apex and Nodal segment explant of pomegranate were placed on Murashige and Skoog Medium (MS medium, 1962) supplemented with different concentration of cytokinins and auxins for callus induction. Antioxidants viz: activated charcoal, ascorbic acid, citric acid and polyvinylpyrollidone were used to control the accumulation of phenolic compounds in the culture medium and enhance the rate of micropropagation. In the present investigation efforts were made to assess the role of antioxidants to minimize the release of phenolic substances at most responsive levels worked out in the present investigation that is maximum shoot bud induction in nodal segment at 2.0 mg/l BAP at activated charcoal (200 mg/l) and callus proliferation in leaf explant at 1.5 mg/l BAP at 200 mg/l of activated charcoal.