Vol. 11, Issue 10 (2022)

Aloe vera is medicinal herb growing in tropical and subtropical latitudes with very good economic potential. It has been used as botanical medicines in many countries for thousands of year, they are used for preparing food, feed, and beverages, formulating ethno medicinal and ethno veterinary remedies and preparing traditional and modern cosmetic products. An efficient protocol for rapid in vitro propagation of valuable medicinal plant Aloe vera by using shoot tip and nodal cutting as explants were done. Brahmi and Aloe vera is likely to become a major source of a number of medicinal products of high value in the coming future. It is imperative to convert Aloe barbadensis from wild plant to plant suited to meet the huge demand of the industries. Aloe vera is the best known today for its ability to treat burns; it has been used for treating stomach disorders, headache, constipation, influenza and fever, colic, kidney ailments, ringworms skin, hemorrhoids, wounds, dystrophy, blistering, sunburn, menstrual problems, insomnia, snakebite, hair loss, meningitis and other elements. The present work deals with the establishment of a viable protocol for this valued medicinal plant. Out of ten different treatments regime tried for induction and multiplication on shoots explants^-1. Taken two types of media i.e. MS media and B5 media supplemented with different doses of plant growth regulators i.e. 1 mg/L of BAP, 2 mg/L BAP, 3 mg/L BAP, 4 mg/L BAP and 5 mg/L BAP for shoot induction and data to be recorded at weekly interval from first week to six weeks. Resulted that media MS was given best response at weekly interval, supplemented with different doses of plant growth regulators (i.e. 1 mg/L of BAP, 2 mg/L BAP, 3 mg/L BAP, 4 mg/L BAP and 5 mg/L BAP) for shoot induction per explants of Aloe vera as compare to B5 media. Doses 3.0 mg/l BAP supplemented in MS media and B5 media was found best to induce maximum number of shoots per explants followed by 4.0 mg/l BAP in Aloe vera. Explants nodal cutting was done highly shoot proliferation with MS media and doses of plant growth regulators as compare to shoot tip explants of Aloe vera.