Vol. 11, Issue 10 (2022)

Author(s):
Nudrat Sanzida Akhtar, Mohan Kumar Biswas and Dayaram

Abstract:
Chemical and UV-ray mutagenesis of basidiospores of Lentinula edodes generated new mushroom strains. The basidospores of parent strains (LE-1501, LE-1503, LE-1504 and LE-1505) were treated with ethyl methanesulfonate, an alkylating agent, to yield 16 mutant monokaryotic mycelia. Eight fast-growing mycelia were selected and mated each other by hyphal fusion. Five (Spore pair 4-1, 7-1, 5-3, 6-5 and 8-6) out of the 28 mattings were successful considered by the formation of clamp connections. For UV mutation, Seven days old culture of parent strains were inoculated then the plates were exposed to UV radiation with the help of G30T8 Germicidal 3FT 30W T8 UVC, Philips, under a laminar air flow hood for four different periods i.e. 15, 30, 45 and 60 minutes. Six UV mutant strains signify UV-1, UV-2, UV-3, UV-4, UV-5 and UV-6 with higher radial growth than parent were selected for further studies. The mutant dikaryons were cultivated to investigate their morphological and cultivation characteristics. Most of the mutant strains observed different shape and size of the sporophore from their parent. Mutant strains SP 7-1, SP 6-5, UV-3 and UV- 5 showed another interesting phenotype i.e. white to light straw coloured pileus which is totally different from parents.