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Vol. 10, Issue 9 (2021)

Immunomodulatory effect of vitamin e in Wstar albino rats (Rattus norvegicus)

Author(s):
Dr. K Nagaraja, Dr. Sanganagowda Koppad, Dr. U Sunilchandra, Dr. NB Shridhar, Dr. CR Santhosh and Dr. BM Chandranaik
Abstract:
Twenty four Wistar Albino rats an age of five to six month and weighing 100 to 200 g body weight were randomly divided into four experimental groups each group comprising of six rats. The group I given distilled water and group II given Vitamin E 200 µg/kg body weight administered orally were normal non-antigen stimulated groups, in these groups, group I served as control. The group III given antigen 0.4 ml intraperitoneal and distilled water and group IV given antigen 0.4 ml intraperitoneal plus Vitamin E 200 µg/kg body weight administered orally were antigen stimulated groups, in these, Group III which was given only antigen and distilled water, served as antigen control group. All these groups were fed with pelleted feed and given water ad libitum. They were maintained hygienically under standard laboratory conditions. The rats were allowed to get accustomed to laboratory environment for a week before the start of the experiment. The blood was collected from all the groups on day zero, i.e., immediately before administering distilled water and drug and then on the 14, 20, 28, 35 and 42 days after treatment. In non-antigen stimulated group II highest immunoglobulin concentration was seen on day 35 and in antigen stimulated group IV the highest immunoglobulin concentration was found on day 28. But There was no significant (P>0.05) difference in the mean total serum immunoglobulin concentration of normal non-antigen and antigen stimulated groups when compared with their respective control groups. But in both normal non-antigen stimulated and antigen stimulated groups which were given Vitamin E alone and Vitamin E plus antigen, did not produce any significant change in the values of TIG, TSP, SA, TSG, A:G ratio and DNCB induced skin thickness when compared with their respective control groups. But There was a significant (P< 0.05) increase of TLC values in group II from day 35th to 42nd and in the group IV the TLC values increased on day 20, 28, 35 and 42, compared to their respective non-antigen and antigen control groups (Group I and III). There was a significant (P< 0.05) increase of ALC values in both group II and IV compared to their respective control groups (Group I and III), the increase was seen from 35th to 42nd day. Also there was a significant (P< 0.05) increase of PI values in group II and IV compared to their respective control groups, the increase was seen from 28th to 42nd day.
Pages: 1639-1645  |  219 Views  61 Downloads


The Pharma Innovation Journal
How to cite this article:
Dr. K Nagaraja, Dr. Sanganagowda Koppad, Dr. U Sunilchandra, Dr. NB Shridhar, Dr. CR Santhosh, Dr. BM Chandranaik. Immunomodulatory effect of vitamin e in Wstar albino rats (Rattus norvegicus). Pharma Innovation 2021;10(9):1639-1645.

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