Vol. 10, Issue 9 (2021)

Author(s):
V Ramakrishnan, A Arivuchelvan, M Parthiban, M Thangapandiyan and SP Preetha

Abstract:
The present study was designed to find potential target protein for fucoidan against Colon cancer in rats. The sequence of all the seven target proteins were retrieved from UniProt database. As the 3D structure is not available for these target proteins, the sequence of all the seven target proteins were modelled using Swiss-Model and the results were evaluated using Ramachandran Plot. The 3D structure of the compound fucoidan was obtained from PubChem database. All the target proteins were subjected to perform docking studies with fucoidan. The PyRx software was used to carry out this docking studies. Discovery studio 2021 was used to analyse the results. From results, the target protein RAC-alpha serine/threonine-protein kinase (Akt1), Vascular endothelial growth factor receptor 2 (Kdr) and Phosphatidylinositol 3-kinase catalytic subunit type 3 (Pi3k) showed very good binding affinity of -6.1, -5.8, -5.6 Kcal/mol, respectively with the compound fucoidan. Hence, the present study concludes that the best binding affinity was obtained between RAC-alpha serine/threonine-protein kinase and fucoidan and finally this study suggests that RAC-alpha serine/threonine-protein kinase belongs to Akt1 gene may act as a potential target protein for fucoidan.