Vol. 10, Issue 6 (2021)

Author(s):
TR Shaikh, KM Sharma and GS Pawar

Abstract:
Sorghum is one of the main staple food for the world’s poorest and most food insecure people across the semi-arid tropic. It is the second crop after maize in developing high yielding hybrids using cytoplasmic-genetic male sterility system (Arya et al., 2014). In this context, determination of genetic purity is an essential requirement for its commercial success of the hybrid. In present investigation, four sorghum hybrids were screened with RAPD and SSR markers for hybrid confirmation and purity testing. Five RAPD primers and eight SSR markers enabled to assess the purity of hybrid with their parents. Among RAPD, the primer OPA-4 produced maximum of 100% polymorphism while primer OPA-18 showed 71.43% polymorphism and found to be least polymorphic. Also, the PIC values of eight polymorphic SSR markers ranged from 0.44 to 0.77 with an average of 0.58. The microsatellite Sb6-84 was observed to be most informative with the PIC value of 0.77, which significantly determined genetic relatedness among hybrids and parental lines. Sb4-121 alone could confirm three hybrids by producing male parent specific allele. The genetic similarity retrieved on the basis of combined RAPD and SSR data using Dice coefficient was ranged from 0.31 to 0.87 with an average of 0.53. The dendrogram developed through UPGMA clustered the hybrids with their respective male parent revealed similarity among them being the true hybrids.