Vol. 10, Issue 5 (2021)

Author(s):
Sathish Kumar JK, Vinothkumar B, Paramasivam M and Arulkumar G

Abstract:
A simple, reliable and sensitive reverse-phase high-performance liquid chromatography method with photodiode array detector was developed and validated for analysis of the dislodgeable foliar residues of chlorantraniliprole on maize leaf. Chromatographic conditions for reversed - phase HPLC with photodiode array detection were as follows: column, C18 (Agilent) column, 25 length x 4.6 cm, 5 μm; column temperature, 40 °C; mobile phase, a 70:30 (v/v) acetonitrile: water; flow rate 1 mL/min. The detection wavelength was UV 254 nm. A good linear relationship R² = 0.999 was obtained for chlorantraniliprole from 0.05 to 3.2 μg/ml. The limit of detection (LOD) and the limit of quantification (LOQ) were 0.007 ng/cm² and 0.023 ng/cm² respectively. Recoveries were 94.53 ± 0.70% and precision were 0.74 to 2.58%. The proposed method was successfully applied to analysis of the dislodgeable foliar residues on maize leaf.