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- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 10, Issue 5 (2021)
Extract from Endophytic Bacillus subtillis and Its Mechanistic Effect on Chickpea Dry Root Rot Causing Pathogen Rhizoctonia bataticola (Taub.) Butler
Author(s):
N Chiranjeevi, M Reddi Kumar, B Padmodaya, NC Venkateswarlu, P Sudhakar, R Sarada Jayalakshmi Devi and P Aruna Sri
N Chiranjeevi, M Reddi Kumar, B Padmodaya, NC Venkateswarlu, P Sudhakar, R Sarada Jayalakshmi Devi and P Aruna Sri
Abstract:
In the present study, crude metabolic substances from endophytic bacteria Bacillus subtillis were extracted by solvent extraction method and tested their bio-efficacy against R. bataticola by agar well diffusion assay. Among the different concentrations tested significantly higher inhibition was recorded at 100 μl (77.23 %). The microscopical examination of the crude metabolite treated mycelia of the R. bataticola revealed its effect on the fungal morphology. Light microscopic analysis showed that severe mycelial destruction such as breakages, vacuolations, bursting, swellings, lysis, bulged hyphal tips and hyphae, cytoplasmic aggregations etc., and the SEM images clearly explained the disruption of the fungal hyphae due to the mechanistic effect of antibiotic compounds on fungal cell wall.
In the present study, crude metabolic substances from endophytic bacteria Bacillus subtillis were extracted by solvent extraction method and tested their bio-efficacy against R. bataticola by agar well diffusion assay. Among the different concentrations tested significantly higher inhibition was recorded at 100 μl (77.23 %). The microscopical examination of the crude metabolite treated mycelia of the R. bataticola revealed its effect on the fungal morphology. Light microscopic analysis showed that severe mycelial destruction such as breakages, vacuolations, bursting, swellings, lysis, bulged hyphal tips and hyphae, cytoplasmic aggregations etc., and the SEM images clearly explained the disruption of the fungal hyphae due to the mechanistic effect of antibiotic compounds on fungal cell wall.
Pages: 898-905 | 537 Views 285 Downloads
How to cite this article:
N Chiranjeevi, M Reddi Kumar, B Padmodaya, NC Venkateswarlu, P Sudhakar, R Sarada Jayalakshmi Devi, P Aruna Sri. Extract from Endophytic Bacillus subtillis and Its Mechanistic Effect on Chickpea Dry Root Rot Causing Pathogen Rhizoctonia bataticola (Taub.) Butler. Pharma Innovation 2021;10(5):898-905.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
