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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 10, Issue 5 (2021)
In-vitro propagation of Nagpur mandarin (Citrus reticulata)
Author(s):
Bodade SR, Gahukar SJ and Akhare AA
Bodade SR, Gahukar SJ and Akhare AA
Abstract:
The present study was undertaken with an objective to develop a protocol for micropropagation of Nagpur mandarin (Citrus reticulata) through shoot tip. Shoot tip of 1 to 2 cm were collected and culture in Murashige and Skoog (MS) medium. The treatment HgCl2 0.1% for 10 min was found to be best sterilization treatment with respect to survival culture percent and aseptic culture percent. BAP 2mg/l was found to be best treatment for establishment media with respect to maximum sprouting, minimum days taken for sprouting with highest number of shoots/explant. IBA 2mg/l + NAA 0.1mg/l + activated charcoal 1000mg/l was found to be significantly superior over all other treatments with respect to maximum root initiation percentage, days to root initiation, highest number of roots.
The present study was undertaken with an objective to develop a protocol for micropropagation of Nagpur mandarin (Citrus reticulata) through shoot tip. Shoot tip of 1 to 2 cm were collected and culture in Murashige and Skoog (MS) medium. The treatment HgCl2 0.1% for 10 min was found to be best sterilization treatment with respect to survival culture percent and aseptic culture percent. BAP 2mg/l was found to be best treatment for establishment media with respect to maximum sprouting, minimum days taken for sprouting with highest number of shoots/explant. IBA 2mg/l + NAA 0.1mg/l + activated charcoal 1000mg/l was found to be significantly superior over all other treatments with respect to maximum root initiation percentage, days to root initiation, highest number of roots.
Pages: 314-316 | 741 Views 374 Downloads
How to cite this article:
Bodade SR, Gahukar SJ, Akhare AA. In-vitro propagation of Nagpur mandarin (Citrus reticulata). Pharma Innovation 2021;10(5):314-316.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
