Vol. 10, Issue 3 (2021)

Author(s):
Shoriful Islam Sagor, Golam Mostofa Sajol, AKM Khurshid Alam and Adeeba Anjum

Abstract:
This study was carried out for the first time to evaluate the antioxidant properties and total phenolic and flavonoid content of the methanolic extract along with its organic and aqueous soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh. For evaluation of antioxidant properties DPPH scavenging assay, ferric reducing capacity, hydroxyl radical scavenging assay and phospho molybdenum assay was used. In the DPPH free radical scavenging assay, the ethyl acetate soluble fraction of the crude methanol extract revealed the highest free radical and hydroxyl radical scavenging activity with IC50 value of 3.22 ± 0.17 μg/ml and 12.88 ± 0.305 μg/ml, respectively while the standard BHT was 4.39 ± 0.02 μg/ml and 9.38 ± 0.075 μg/ml, respectively. All the test samples and the standard BHT exhibited good linear relationship in the ferric reducing capacity and phospho molybdenum assay. Also, the test samples showed significant activities in the ferric reducing capacity and phospho molybdenum assay compared to the reference standard in a dose dependent manner. It was observed that the test samples contained considerable amount of bioactive compounds including total phenolic (ethyl acetate soluble fraction giving the highest 22.631 ± 0.085 GAE/gm of dried sample) and flavonoid (pet ether soluble fraction giving the highest 54.513 ± 1.500 CE/gm of dried sample) content. It can be concluded from the results that the crude methanolic extract along with it’s four soluble fractions of the whole stem of Coffea benghalensis growing in Bangladesh are a good source of natural antioxidants.