Genetic diversity assessment in mango ginger (Curcuma amada Roxb.) germplasm based on SSR and RAPD molecular markers
Kalpesh G Raval, RK Patel, RJ Panchal, KG Modha and GD Vadodariya
During the kharif-2018, the current study was conducted out in the molecular lab of the Department of Genetics and Plant Breeding, Navsari Agricultural University, Navsari (Gujarat). The experimental materials comprised of thirty different genotypes which were assessed with SSR and RAPD markers to assure the genotype divergence. The experiment's main goal was to see how much molecular variation there was in the available mango ginger germplasm, which will serve as base for future mango ginger improvement programmes. For molecular characterization, 9 SSR primer pairs were exploited in mango ginger accessions revealed all were monomorphic and hence the work was altered to RAPD. Furthermore, ten RAPD primers were tested and revealed a significant amount of polymorphism among the thirty mango ginger genotypes, with the best showing 100% polymorphism and high PIC values. The UPGMA algorithm was used to create a dendrogram based on the banding pattern of RAPD markers. The similarity coefficient can be anything between 0.43 and 1.00. The dendrogram clearly separated the thirty genotypes into two main clusters, each of which was further subdivided into eight sub-clusters, demonstrating the genotypic diversity of the genotypes investigated. The findings revealed a link between morphological features and the dendrogram produced by RAPD analysis.
How to cite this article:
Kalpesh G Raval, RK Patel, RJ Panchal, KG Modha, GD Vadodariya. Genetic diversity assessment in mango ginger (Curcuma amada Roxb.) germplasm based on SSR and RAPD molecular markers. Pharma Innovation 2021;10(11):65-71.