Vol. 10, Issue 11 (2021)

Author(s):
Harsha R, Raviraja Shetty G, Rajasekharan PE, Rajeshwari Durgad S, Ganapathi M and Ravi CS

Abstract:
Pollen storage studies in Oroxylum indicum (L.) Vent. was conducted at “In vitro and Cryopreservation laboratory”, Division of Floriculture and medicinal crops, ICAR-Indian Institute of Horticultural Research, Bengaluru during 2020-21. Fresh pollens from 12 different accessions of O. indicum were collected from the Field Gene Bank of RET medicinal plants, IIHR, Bengaluru and pollens were preserved in different storage temperatures viz., 5 °C, -20 °C and -196 °C for 6 months. Initial viability assessment and post storage viability after retrieval was evaluated. The maximum pollen germination percentage was recorded in cryopreserved pollen after six months of storage. The fresh pollen viability was recorded and highest (98.25±0.25%) was noticed in Accession ASRET-2 where cryopreserved pollen recorded germination percentage of 86.65±0.45.