Vol. 10, Issue 11 (2021)

Author(s):
Nandish MS, Vidya MD and Suchitha Y

Abstract:
In an attempt to isolate, characterize, screen and evaluate the native liquid Plant Growth Promoting Rhizomicrobial (PGPR) consortia on growth and yield of Radish. As many as 11 weed rhizosphere samples were collected and used for isolating the beneficial plant growth promoting rhizomicroorganisms using standard serial dilution plate technique. Further based on in vitro studies results, the efficient nitrogen fixing, phosphate and potassium solubilizing isolates were selected and used for developing liquid formulation for evaluation on radish under greenhouse condition. Out of 11 different weed rhizosphere soils collected for the study, as many as 7 Azotobacter, 6 phosphate solubilizing and 8 potassium solubilizing isolates were obtained. In the in vitro screening studies, the Azoto – 7, PSB – 2 and KSB - 8 showed high efficiency in nitrogen fixation, phosphorus and potassium solubilizing ability, and all the efficient isolates were tentatively identified as Azotobacter, phosphate solubilizing Bacillus sp. and potassium solubilizing Bacillus sp.. Further, the efficient liquid plant growth formulation (Azoto - 7, PSB - 2 and KSB - 8) was developed and evaluated on Radish under greenhouse condition using varied levels of Recommended Dose of Fertilizers (RDF). Out of 16 treatments imposed, the treatment receiving 75% of RDF + FYM + efficient liquid PGPR formulation showed statistically highest germination percentage (100%) and number of leaves (15.70, 18.60 and 20.20 numbers of leaves at 15, 30 and 45 days after sowing). Similarly, the same treatment showed high chlorophyll content of 2.90 mg/g of tissue, fresh and dry weight of 141.10 and 29.90 g respectively followed by the treatment receiving 100% of RDF + FYM + efficient liquid PGPR formulation. However, the same trend of observation was recorded with respect to tuber girth, tuber length and also showed the maximum accumulation of NPK content both in plant and soil at the time of harvest.