Vol. 9, Issue 8 (2020)

Author(s):
K Venkata Ramesh, R Sarada Jayalakshmi Devi, K Gopal, BKM Lakshmi and T Murali Krishna

Abstract:
A total of forty isolates of Colletotrichum capsici were isolated from diseased samples and identified based on conidial morphology, size, colour, acervuli and setae. Isolates were designated as Cc 1 to Cc 40. Molecular variability among the isolates of C. capsici was studied using RAPD primers. The RAPD allelic patterns were divided the isolates of C. capsici into three clusters (I-III) in the phylogenetic tree dendrogram at 0.49 co efficient of similarity. The analysis of RAPD polymorphism in isolates of C. capsici from different regions revealed the occurrence of high level of polymorphism, indicating a wide and diverse genetic base. The topology of the dendrogram suggested that most isolates were more than 20% different from each other, and this indicated the existence of both local and geographical polymorphism among the isolates.