Vol. 8, Issue 5 (2019)

Author(s):
Bajad PN, Pardeshi AB and Pagore VP

Abstract:
The isolation of saponin from various solvent extract of the plant, Dodonaea viscose were studied. Dodonaea viscosa is a shrub belonging to the family Sapindaceae, and is commonly called as hop bush. Saponins are heterosides of plant origin and the type of molecules has an interesting pesticide potential. The saponin was isolated from the leaves, which is further purified with the help of solvent separation method and that shows the positive result for preliminary tests such as foam test, Lieberman’s test. The isolated extract was purified by analytical method (Edeoga, et al.). The IR studies of saponin shows the peaks at-3069 (Aromatic –CH stretching), 2924 (-CH₃), 1636 (C=C Stretching), 1076 (C-O-C stretching of glycoside linkage of Oligosaccharide to sapogenin) which indicated the presence of functional group. The HPLC studies of saponin shows the peak at 205nm which get matched with the std. saponin and on the basis of that data reveals both qualitative and quantitative analysis of saponin. The amount of saponin present in Dodonaea viscose was found to be 173.25µg/mg.