Phytochemical analysis and evaluation of antioxidant activity of Premna latifolia Roxb. A medicinal plant (Family: Lamiaceae)
Pushpa Ruwali and Diksha Negi
Traditional medicine employing a plant-based system of treatment forms an important part of human medicine system. The most common reason for utilizing traditional medicine is its non- toxic nature, closely resembles the patient ideology and allays concerns about the adverse effects of chemical medicines, satisfies the desire for more personalized health care, and allows greater public access to health information. Plants and their bioactive principles have a long history of use in modern medicine and in certain systems of traditional medicine. Plant-derived substances have recently become of great interest owing to their versatile applications. The bark and leaves extract of Premna latifolia Roxb. with different solvents were investigated for their phytochemical and antioxidant potential. The phytochemical composition was carried on the bark and leaves extracts of Premna latifolia, revealed the presence of active ingredients such as Glycosides, Steroids, Saponins, Phenols, Flavonoids, Terpenoids, and Tannins. The bark and leaves extracts were also evaluated for antioxidant activities by DPPH radical and ABTS scavenging assay. Among four different solvents used, the maximum antioxidant activity found in methanolic extract followed by hexane, ethanol, and chloroform. The present study reveals that this plant is of therapeutic potential due to their high free radical scavenging activity. The methanolic extract of bark and leaves of Premna latifolia showed the presence of maximum phytochemicals (12&11) when compared to other solvent extracts viz. hexane (PLHEX) (10&9), ethanol (PLETH) (9&8), and chloroform (PLCHLO) (8&7) respectively. The amount of phenolics and flavonoids present in solvents were in the order of Methanol > Hexane > Ethanol > Chloroform. The best antioxidant potential was found in Methanolic extract of Premna latifolia (PLME). Methanolic extract of both bark and leaves gave an IC50 (135.53μg/ml &178.11μg/ml) for DPPH (2, 2-diphenyl-2-picryl hydrazyl) radical scavenging assay and (102.05μg/ml &111.26μg/ml) for ABTS (2, 2’-azinobis-3-ethyl-benzothiozoline-6-sulphonic acid) radical scavenging assay. This study shows that the bark and leaves of Premna latifolia extract could be used as a probable antioxidative agent. This study also revealed that Premna latifolia bark possesses greater antioxidant potential in comparison to leaves.
How to cite this article:
Pushpa Ruwali, Diksha Negi. Phytochemical analysis and evaluation of antioxidant activity of <em>Premna latifolia</em> Roxb. A medicinal plant (Family: Lamiaceae). Pharma Innovation 2019;8(5):13-20.