Phytochemical screening and quantitative estimation of total flavonoids of Ocimum sanctum in different solvent extract
Praveen Garg and Rajesh Garg
Ocimum sanctum (Tulsi) is important and widely used medicinal plant. It is an aromatic plant of the lamiaceae family. The aim of present study is to estimate the phytochemical components and total flavanoid content assay of Chloroform, methanol and ethanolic solvent extract from basil leaf and stem. The basil leaf and stem was extracted by maceration process using ethanol, chloroform, and methanol. The Chloroform, ethanol and methanol extract were screened of phytochemical content including identification of flavonoid, alkaloid, polyphenols, glycosides, tannin, saponin etc. Estimation of total flavonoids content was based on aluminium chloride method in the sample extract by spectrophotometrically. Phytochemical screening test showed that the presence of diterpines, saponins, proteins, flavonoids, amino acids, carbohydrates, alkaloids in leaves and stem parts when extracted with methanolic and ethanolic solvents. Chloroform extract of basil leaf and stem does not show the presence of any phytochemicals. Higher flavonoid component were present in methanolic extract of Ocimum sanctum leaves than ethanolic solvent extract. In this study, Ocimum sanctum has phytochemicals properties in the leaves and stem which are used in curing the ailments and higher flavonoid content indicated the natural antioxidant activity signifying their medicinal importance and potent source in pharma industries. We conclude that Ocimum sanctum is highly useful medicinal plant. However, there is necessary to explore natural plant sources with their medicinal value used in medical field.
How to cite this article:
Praveen Garg, Rajesh Garg. Phytochemical screening and quantitative estimation of total flavonoids of <em>Ocimum sanctum</em> in different solvent extract. Pharma Innovation 2019;8(2):16-21.