Vol. 8, Issue 12 (2019)

Author(s):
Srikanth MK, A Gopala Reddy and A Anand Kumar

Abstract:
An experimental study was conducted to evaluate the interaction of Gymnema sylvestre extract with insulin and glimepiride in diabetic Sprague dawley rats. Rats were randomly divided into seven groups of six rats in each and blood glucose was estimated to ascertain group differences. Group I was kept as normal control. Remaining six groups were induced diabetes by intra peritoneal injection of streptozotocin at the rate of 40 milligram per kilogram body weight. After 72 hours, rats with blood glucose value of more than 200 milligram per decilitre were included in the study. Treatment protocols were initiated 48 hours post-confirmation of diabetes and continued for 2 months. Group 1 is non-diabetic control, group 2 is streptozotocin (40 milligram per kilogram intra peritoneal single dose)-induced diabetic control, group 3 is insulin treatment(4 Units/ kilogram body weight subcutaneously once daily), group 4 is glimepiride treatment (4 milligram per kilogram b.wt orally once daily), group 5 is Gymnema sylvestre methanolic leaf extract treatment (400 mg/kg body weight orally once daily), group 6 Insulin + Gymnema sylvestre methanolic leaf extract treatment (once daily) and group 7 is glimepiride + Gymnema sylvestre methanolic leaf extract treatment(once daily). A detailed necropsy was conducted on each rat and kidney, pancreas and aorta were collected for ultra structural studies. The electron microscopy of kidney, pancreas and aorta revealed marked alterations in group 2, while group 6 and 7 revealed better architecture.