Vol. 7, Issue 8 (2018)
Phosphatidylcholine attenuates LPS-induced immunomodulatory effect through slc26a3/DRA-mediated signaling in primary chicken intestinal epithelial cells
Vimal Manivannan, Yogalakshmi Sabapathy, Harini Challa, Thiyagarajan Sanjeevi and Umadevi Subramanian
The present study was aimed to study the protective effect of Phosphatidylcholine (PC) on lipopolysaccharids (LPS)- induced oxidative stress in chicken intestinal epithelial cells and to investigate the possible role of SLC26A3 (the ion exchanger) on immune augmentation. Intestinal epithelial cells from broiler chicks were cultured in Dulbecco’s modified Eagle’s medium/F12, exposed to PC and LPS. The levels of oxidative damage, antioxidants, cellular proliferation, pro-inflammatory and anti-inflammatory cytokines expression levels were assessed. Further, SLC26A3/DRA mediated signaling was elucidated by silencing DRA in vitro. On incubation with LPS, cells showed an increase in MDA levels, with increased pro-inflammatory cytokines including TNF-α, IL-6, IL-1β, IL-2 and decreased anti-inflammatory cytokines IL-4, IL-10, IL-13, and TGF-β levels compared to control. In contrast, PC treated cells improved the antioxidant levels with increased anti-inflammatory and decreased pro-inflammatory cytokines in intestinal epithelial cells. Mechanistically, siRNA knockdown of DRA showed the decreased anti-inflammatory cytokines (p<0.01) and increased pro-inflammatory cytokine (p<0.01) in cells compared to control. These results demonstrate that PC exerts protection in primary chicken intestinal epithelial cells from oxidative damage and stimulating anti-inflammatory cytokines mainly through DRA activation suggest that PC might be provided as a feed supplement to improve anti-inflammatory ability of chicken.
How to cite this article:
Vimal Manivannan, Yogalakshmi Sabapathy, Harini Challa, Thiyagarajan Sanjeevi, Umadevi Subramanian. Phosphatidylcholine attenuates LPS-induced immunomodulatory effect through slc26a3/DRA-mediated signaling in primary chicken intestinal epithelial cells. Pharma Innovation 2018;7(8):73-81.