Vol. 7, Issue 7 (2018)

Author(s):
Mohita Rai and Aditya Mishra

Abstract:
In both methods, the detection involved sandwiching of the target AIV between monoclonal antibodies for nucleoproteins and for matrix proteins. In the fluorescent DNA barcode-based immunoassay, fluorophore-tagged oligonucleotides were used as surrogates for signal detection with sensitivity comparable to conventional RT-PCR for allantoic fluid containing AIV. While in the fluorescent bead-based immunoassay, the fluorescent beads were used as the direct detection signal from AIV.