Evaluation of mast cell stabilizing activity of Camellia sinensis and Ocimum basilicum and correlation with their antioxidant property
Manjot Kaur, Varinder Singh and Richa Shri
Mastocytes are vital for human immunity. Mast cell degranulation leads to several immune/allergic reactions. A major factor responsible for mast cell disruption is oxidative stress. Hence antioxidants may play a significant role in mast cell stabilization. Antioxidant plants such as Camellia sinensis (Theaceae) and Ocimum basilicum (Lamiaceae) are used in allergic conditions in traditional medicine. However, a little is known about their mast cell stabilizing potential. The present study evaluated the mast cell stabilizing activity of two polyphenol rich plants viz. C. sinensis and O. basilicum and correlated this with their antioxidant capacity. Acetone and hydroalcoholic extracts of the two plants were prepared and standardized with respect to total phenolic and flavonoid content. Their antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay. Mast cell stabilization was assessed by the ability to inhibit histamine release in an in vitro model that employs compound 48/80 (a potent mast cell activator) to induce rat peritoneal mast cell degranulation. Acetone extract of C. sinensis showed significant free radical scavenging activity (IC50 value 8 µg/ml) and the potent inhibition (>80%) of histamine release at a concentration of 100 µg/ml. Significant positive linear correlation (correlation coefficients <1) for all extracts suggests that antioxidant activity contributes to mast cell stabilization. Management of oxidative stress may aid in preventing histamine release possibly by reducing mast cell degranulation. The strongly antioxidant acetone extract of C. sinensis may be a potential mast cell stabilizer.
How to cite this article:
Manjot Kaur, Varinder Singh, Richa Shri. Evaluation of mast cell stabilizing activity of Camellia sinensis and Ocimum basilicum and correlation with their antioxidant property. Pharma Innovation 2018;7(3):69-73.