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Vol. 7, Issue 12 (2018)

Development and validation of a HPTLC method for simultaneous estimation of quercetin, Chlorogenic acid and trigonelline in polyherbal antibacterial formulation

Author(s):
Shiv K Yadav, Gaurav Jain, Avijit Mazumder and Roop K Khar
Abstract:
The overall aim of the present study is development of simultaneous quantification of Quercetin QC, Chlorogenic Acid (CA) and Trigonelline (TN). The literature reports wide array of analytical techniques for the qualitative and quantitative determination of these biomarkers individually and also in combination with other compounds, no reports are available till date for the simultaneous quantification of QC, CA and TN. The UV absorption spectra of the three biomarkers at different wavelengths were recorded by multi-wavelength detector, the QC, CA and TN showed λmax of 290 nm, 330 nm, 270 nm in absorbance mode, respectively. The simultaneous detection and co-quantification of all three compounds was successfully achieved for the first time. The developed and validated HPTLC method for simultaneous determination of QC, CA and TN is rapid, reliable and low cost.
Pages: 75-81  |  520 Views  20 Downloads
How to cite this article:
Shiv K Yadav, Gaurav Jain, Avijit Mazumder, Roop K Khar. Development and validation of a HPTLC method for simultaneous estimation of quercetin, Chlorogenic acid and trigonelline in polyherbal antibacterial formulation. Pharma Innovation 2018;7(12):75-81.
The Pharma Innovation Journal