Optimization of low-cost substrate for the production of single cell protein using Kluyveromyces marxianus
Mohammed Nayeem, Komal Chauhan, Saif Khan, Gurdeep Rattu, Rohant Kumar Dhaka and Hamda Sidduqui
Advent of 21st century has brought an exponential growth in population thereby generating a great threat to food availability and agriculture especially in less developed countries. Single cell protein (SCP) production at a large scale is believed to be a pragmatic solution to the problem of global food shortage. The present work aims at optimizing the growth conditions and identifing a low cost substrate for the production of SCP using Kluyveromyces marxianus. K. marxianus is known to have a wide range of application in food industries and in the field of biotechnology. The parameters selected to test the optimum growth conditions were pH, temperature, varied combination of carbon and nitrogen sources. It was found that the strain of K. marxianus recorded maximum growth at pH 7 and temperature of 30°C. Three carbon sources i.e. lactose (4%), maltose (4%) and glucose (4%) and three nitrogen sources i.e. ammonium nitrate (1%), di-ammonium hydrogen phosphate (1%) and yeast (0.5%) + peptone (0.5%) were tested in all possible combinations and the maximum growth results was obtained with the combination of lactose and yeast extract + peptone. Whey was identified as a cost effective raw material for the production of SCP and uninterrupted fermentation was carried out under laboratory conditions wherein 80% of biomass and protein content was harvested within 24 h. At this time the biomass yield was 36 mg/ml in which the crude protein content was 83.33%.
How to cite this article:
Mohammed Nayeem, Komal Chauhan, Saif Khan, Gurdeep Rattu, Rohant Kumar Dhaka, Hamda Sidduqui. Optimization of low-cost substrate for the production of single cell protein using Kluyveromyces marxianus. Pharma Innovation 2017;6(8):22-25.