Vol. 6, Issue 12 (2017)
Comparative alpha amylase and alpha glucosidase inhibitory activities of Nishamalaki, Amalaki rasayana-an ayurvedic formulations
Prashant B Bedarkar
Powder of fruit pulp of Indian gooseberry (Amalaki) and Turmeric levigated multiple times with juice of Amalaki i.e. Amalaki swarasa bhavita Nisha Amalaki churna (ASBNAC) is commonly called as Nishamalaki (NA) which is recommended in Ayurvedic classics and is widely practiced for treatment and progression of Diabetes (Madhumeha) and its complications. Previous studies on Nishamalaki also suggest possibility of additive or synergistic effect among 2 drugs Turmeric and Amalaki when formulated in to a compound formulation. Although Alpha amylase and alpha glucosidase activities of Nishamalaki and Amalaki rasayana both prepared by levigation method have been studied, but still comparative study of these activities of Nishamalaki and its formulation components, Amalaki rasayana and Turmeric powder at different doses have not been evaluated to evaluate synergistic effect in a single study hence, its in-vitro study was conducted to evaluate possible synergistic effect due to levigation process. Nishamalaki yoga (NA) and Amalaki Rasayana were prepared with 16 times lavigation (Bhavana) of combination of fine Turmeric powder [Haridra Churna (HC)] and fine powder of fruit pulp of goose berry (Amalaki churna) in equal quantity with half quantity of fresh fruit pulp juice of Amalaki (Swarasa) as that of combined powder for each levigation for preparation of Nishamalaki and levigation with equal quantity of Juice to Powder of gooseberry (as that of later) for preparation of Amalaki Rasayana followed by drying. Methanolic extracts of Nishamalaki, Amalaki rasayana, Turmeric powder and powder of Emblica officinalis were tested for Porcine pancreatic a- amylase inhibition and intestinal a- glucosidase activities at 4 concentrations (40, 80, 120 and 160 μg/ml) through starch as base and chromogenic DNSA (3, 5-dinitrosalicylic acid) as colouring agent, in 2 different solutions. Results and Conclusion: All the tested drugs exhibited comparatively better a- amylase inhibition and a-glucosidase inhibition by acetic acid buffer in the concentration of 40, 80, 120 and 160 μg/ml and inhibition was dose dependent at all concentration thus have potential to reduce post prandial hyperglycemia. Amalaki rasayana showed comparatively better inhibition than same concentration of Nishamalaki or Turmeric powder or powder of Indian gooseberry.
How to cite this article:
Prashant B Bedarkar. Comparative alpha amylase and alpha glucosidase inhibitory activities of Nishamalaki, Amalaki rasayana-an ayurvedic formulations. Pharma Innovation 2017;6(12):424-429.