Level of reactive response of peripheral blood neutrophil granulocytes of patients with acute pancreatitis depending on genes polymorphism of CFTR (delF508C), PRSS1 (R122H), IL-4 (C-590T) and TNF-α (G-308A)
Serhiy Ivashchuk and Larysa Sydorchuk
The neutrophil granulocytes play the key role in the nonspecific anti-infectious protection and adaptive immunity formation. However, the questions of the reactive response of the peripheral blood neutrophils of the patients with edematous pancreatitis even depending on candidate genes polymorphism stay not studied. The aim of our research was to investigate the level of the reactive response of peripheral blood neutrophils in patients with edematous pancreatitis depending on the polymorphism of genes CFTR (ΔF508, rs 113993960), PRSS1 (R122H or G365A, rs 111033565), IL-4 (C-590T, rs 2243250) and TNF-α (G-308A, rs 1800629). 123 patients with edematous pancreatitis were involved in the research. It has been established, that the carriers of NN-genotype of the gene CFTR had higher neutrophils reactive response than such of NM-carriers 3.45 times (p<0,01), neutrophil-to-lymphocyte ratio – 1.44 times (p<0,05), neutrophil granulocytes shift index – 2.93 times (p<0,01), ratio index of leukocytes and erythrocyte sedimentation rate – 12.41 times (p<0,001); organism resistance and leukocyte indexes – 1.55 and 1.85 times (p<0,01) correspondingly, however lymphocyte/granulocyte ratio was higher in NM-carriers 1.36 times (p<0,01). The carriers of GA-genotype of gene PRSS1, in comparison with GG-carriers, had lower nonspecific organism resistance 1.98 times (p<0,001), but higher level of neutrophils reactive response – 22.79-28.65 times (p<0,001), bigger neutrophil-to-lymphocyte ratio – 2.02 times (p<0,01), shift indexes of neutrophils and leukocytes – 8.28 and 3.08 times (p<0,001), ratio index of leucocytes and erythrocyte sedimentation rate and leukocyte index – 5.84 and 7.29 times (p<0,001), with lower lymphocyte/granulocyte ratio 3.63 times (p<0,001). Conclusions. The presence of NN-genotype of gene CFTR, GG polymorphic variant of the gene PRSS1 and GG-genotype of gene TNF-α in a patient with edematous pancreatitis is accompanied by a credibly higher level of non-specific resistance of the organism, neutrophils reactive response, and indicates the superiority of immune response by affective cell type and bigger microphage system activity. In the TT-genotype carriers of the gene IL-4, there is lower activity of the factors and mechanisms of anti-infective defence and general organism resistance.
How to cite this article:
Serhiy Ivashchuk, Larysa Sydorchuk. Level of reactive response of peripheral blood neutrophil granulocytes of patients with acute pancreatitis depending on genes polymorphism of CFTR (delF508C), PRSS1 (R122H), IL-4 (C-590T) and TNF-α (G-308A). Pharma Innovation 2016;5(9):96-100.