Screening mangrove actinomycetes for anticandida activity
T Janaki, BK Nayak and T Ganesan
Candida albicans is the most prevalent pathogenic and causal agent of oral candidiasis in man. Even though many azole based antimycotics are available, still candidiasis persists. The present investigation aims at finding better anticandida compound for controlling the candidiasis. The 25 aerobic mangrove actinomycetes were isolated from the soil collected near the root region of Avicennia marina (Forsk). Vierh - (Avicenniaceae), of Ariyankuppam backwater area. Out of 25, 13 actinomycetes (52%) showed anticandida activity. The most active isolate was identified as Streptomyces cacaoi subsp. cacaoi, Gene bank accession no: KP872910, 100µl culture filtrate shown maximum inhibitory zone measuring as 28mm in well diffusion method. Agar plug method has given inhibitory zone as 32mm. Methanolic partially purified compound fraction of M20 has shown 28mm of inhibitory zone, it was higher than the antibiotic clotrimazole used. The presence of pyrimidine nucleosides - neutral and acidic Polyoxins (230 nm), (270-290 nm) and Heptaene antifungal antibiotics (406-417 nm) are confirmed from the UV-Visible spectral analysis. It was concluded that the pyrimidine and polypeptide nucleoside compounds are responsible for anticandida activity
How to cite this article:
T Janaki, BK Nayak, T Ganesan. Screening mangrove actinomycetes for anticandida activity. Pharma Innovation 2016;5(7):29-35.