In vitro propagation of Orthosiphon stamineus Benth (Lamiaceae) an important medicinal plant using nodal and leaf explants
EV Sheena, Gabriel Jeya Jothi
The present paper reports the in vitro propagation of the valuable medicinal plant,Orthosiphon stamineus Benthin various concentrations of plant growth regulators. Nodes and leaves were used as explants for the induction of multiple shoots and callus respectively. The explants were cultured on Murashige and Skoog’s (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and Kinetin (KIN) alone and BAP in combination with α- Naphthalene acetic acid (NAA) for multiple shoot induction. For callus induction leaf explants were cultured on MS medium supplemented with different concentrations of auxins like 2, 4-Dichlorophenoxyacetic acid (2, 4-D), NAA and Indole-3-butyric acid (IBA). The maximum number of shoots was produced in BAP 1.00mg/l after 8 weeks of culture. An average of 12.9 ± 0.10 shoots measuring 5.94 ± 0.13 mean length were produced from each explants. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of IBA. Highest percentage of root induction was observed in 4.00 mg/L IBA. The rooted plantlets were hardened in the mixture of 1:1:1 ratio of soil, sand and vermicompost. 60-70% plants were successfully established in the field. Optimum callus induction was observed on MS medium supplemented with 2.00 mg/l 2, 4-D.The callus was subcultured on MS medium containing different combinations of BAP and NAA in various concentration. Though the callus showed greenish somatic embryoids, it failed to produce shoots even after 8 weeks of culture.
How to cite this article:
EV Sheena, Gabriel Jeya Jothi. In vitro propagation of Orthosiphon stamineus Benth (Lamiaceae) an important medicinal plant using nodal and leaf explants. Pharma Innovation 2015;4(7):06-10.