Different Spectrophotometric and TLC-Densitometric Methods for Determination of Diclofenac Na and Lidocaine HC
Nourddin W. Ali, Nada. S. Abdelwahab, Mohammed M. Abdelkawy and Ahmed M. Elgebaly
Diclofenac sodium (DIC) Diclofenac Na is a non-steroidal anti-inflammatory drug (NSAID), which is used for relieving pain and inflammation in various conditions, while Lidocaine HCl (LID) is a local anathesetic which acts by preventing the generation and transmission of impulses along nerve fibers and nerve ending. In this work, three accurate, precise and specific spectrophotometric and chromatographic methods have been developed and validated for determination of these drugs in their bulk powder and pharmaceutical dosage form. The developed methods are Ratio subtraction spectrophotometric (Method I), mean centering of ratio spectra MCR (Method II) and TLC-Denitometric (Method III), In method (I) where LID can be determined by dividing the spectrum of the mixture by the spectrum of DIC (as a devisor) followed by subtracting the constant absorbance value of the plateau region, then finaly multiplying the produced spectrum by the spectrum of the divisor, while DIC concentration can be determined directly at 282 nm. In method (II) absorption spectra of each drug were recorded, divided by suitable divisor and the obtained ratio spectra were then mean centered. Method (III) is TLC –Densitometric method that depends on quantitative separation of DIC and LID on TLC plates using ethyl acetate: chloroform: methanol: ammonia (5:3.3:1.5:0.2 by volume) as mobile phase and scanning at 203 nm.The developed methods were validated according to ICH guidelines demonstrating good accuracy and precision. The results were statistically compared with those obtained by reported method and no significant difference were found between them.
Chemical structures of (a) Diclofenac Na, (b) Lidocaine HCl
How to cite this article:
Nourddin W. Ali, Nada. S. Abdelwahab, Mohammed M. Abdelkawy, Ahmed M. Elgebaly. Different Spectrophotometric and TLC-Densitometric Methods for Determination of Diclofenac Na and Lidocaine HC. Pharma Innovation 2014;3(6):57-64.