Volume 3, Issue 12
Production, purification and characterization of bacitracin from Bacillus subtilis
Author: M.Rukmini, Debasish Sahoo, Jikasmita Dalei, Ravitosh Ray
Abstract: Bacillus subtilis capable of producing bacitracin was isolated from soil and screened for the production of bacitracin on nutrient media against test pathogenic organisms viz: Micrococcus luteus, (ATCC 9341) and Staphylococcus aureus (ATCC 13565). Bacitracin was produced by submerged fermentation from the isolated bacteria and checked for their antimicrobial activity by agar well diffusion method. The bacitracin was extracted and purified by Butanol-Ether liquid extraction system and identified by thin layer chromatography. By optimizing the media composition and culture conditions, the effect of different nitrogen sources (Asparagine, Methionine, peptone, yeast extract, Sodium nitrite, Ammonium nitrite), carbon sources (glucose, fructose, sucrose, lactose) pH (5, 6, 7, 8, 9), time (48 hours, 72 hours, 120 hours, 144 hours), temperature (-4 °C, 37 °C, 42 °C and 30 °C) on the production of bacitracin were investigated by agar diffusion assay as detected by the size of zones of inhibition. The maximum production was observed by using glucose as carbon and asparagine as nitrogen source and after incubating at 42 °C of pH 7.0 for 144 hours.
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