Volume 3, Issue 11
Diagnostic significance of apoptosis of peripheral blood lymphocytes in patients with chronic heart failure
Author: N.H.Virstiuk, O.Ye.Cherkashyna
Abstract: The aim of our research has been the study of changes in the apoptosis of peripheral blood lymphocytes in patients with chronic heart failure depending on the stage of the disease.
Materials and Methods: The study involved 86 patients with hypertension complicated by heart failure. There was conducted a general clinical, ultrasound and immunological examination of patients. Determination of apoptosis-mediated factor CD95 (Fas, APO-1) expression on the surface of lymphocytes was performed using an indirect immunofluorescence method with monoclonal antibodies. The state of cellular immunity was assessed by the immunofluorescence method using monoclonal antibodies. The tumor necrosis factor alpha (TNFα) content in the patients blood was determined by the immunoassay method.
Results: In patients suffering from AH with CHF there was revealed of apoptosis activation of peripheral blood lymphocytes in 49 (76.56%) patients with CHF NYHA class III and in 21 (95.46%) patients with CHF NYHA class IV. The apoptosis activation of peripheral blood lymphocytes in CHF patients accompanied by the secondary immunodeficiency. A direct correlation between the increase of pro-inflammatory cytokine TNF-a content in the blood and the increase of expression level of CD95 (Fas, APO-1) on peripheral blood lymphocytes was established (r=0.61; p<0.05).
Conclusion: In patients with CHF the activation of peripheral blood lymphocytes apoptosis was identified, which increases with the CHF stage increase and accompanied by secondary immunodeficiency and increase of TNF-a content in the blood.
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