Vol. 9, Issue 3 (2020)

Author(s):
Divya Dixit, Mohan Lal Jakhar, Sarfraz Ahmad, Suman Chahar, Kassim Yahya Mtilimbanya and Hari Ram Jat

Abstract:
The present investigation was carried out to study effect of different explants on micropropagation of Aloe vera. The experiment was conducted using completely randomized design with ten replications at Tissue Culture Laboratory of S.K.N. Agriculture University, Jobner, India during 2018-19. Auxiliary shoot and apical shoot were used as explants in MS media containing same concentrations of plant growth hormone. The cultures were incubated at 25 ± 2°C under 14:10 hour’s photoperiod with a light intensity of 3000 lux. Among these two explants auxiliary shoot showed significantly higher number of shoots (11.0) with longer shoots length (9.65 cm) compare to apical shoot explant. Also, auxiliary shoot induced higher number of roots (6.8) with longer root length (7.89 cm) than apical shoot explants. Thus for large scale micropropagation, auxiliary shoot explants were significantly better than apical shoot explant for both shoot proliferation and root induction of Aloe vera.