Vol. 8, Issue 8 (2019)

Author(s):
Neha P Singh, Dr. Dulendra P Damahe and Dr. Sachin B Narkhede

Abstract:
A simple, rapid, economical, precise and accurate HPTLC method for simultaneous estimation of Ofloxacin, Ornidazole and Racecadotril has been developed. Chromatographic separation was achieved using silica gel aluminium plate 60F₂₅₄ (10*10) as a stationary phase and Methanol: chloroform: Diethylamine (2.5:7.5:0.1) as a mobile phase. The developed plates scanned densitometrically using UV 254 nm Wavelength. The R_f value of OFL, ORN and RAC was found to be 0.73, 0.44 and 0.36 respectively. The method is validated for different validation parameter such as linearity, accuracy, precision, LOD, LOQ and robustness and the result were found to be within the acceptance limit as per the guideline of international conference on harmonization (ICH).