Vol. 8, Issue 8 (2019)
Multiple anthelmintic resistance in a village sheep flock in Mahendergarh district (Haryana)
Priyanka, Sukhdeep Vohra, Satyavir Singh and Arun K Sangwan
Sixty sheep with egg per gram of more than or equal to 150 were divided into four groups i.e. S1, S2, S3 and S4 of 15 animals each to assess the prevalence of anthelmintic resistance in gastrointestinal nematodes against commonly used anthelmintics in Mahendergarh district (Haryana). Group S1, S2 and S3 were treated with fenbendazole @ 5 mg/kg b.wt. orally, morantel @ 10 mg/ kg b.wt. orally and ivermectin @ 0.2 mg/kg b.wt. subcutaneously, respectively. Group S4 served as untreated control. Faecal egg count was ascertained on day of treatment (0 day) and 12th day post treatment (PT), from sheep of all groups and individual faecal egg counts were determined by the modified McMaster technique. Pooled faecal cultures were made to recover infective larvae on day 0 and 12 PT. Results revealed that fenbendazole reduced the faecal egg counts by 67.19% on 12th day PT with upper and lower confidence levels as 83.26% and 35.69%, respectively, morantel caused 92.11% reduction in faecal egg counts with upper and lower confidence levels as 96.77% and 80.74%, respectively and ivermectin caused 80.44% reduction in faecal egg counts with upper and lower confidence levels as 90.82% and 58.32%, respectively indicating moderate anthelmintic resistance for all three drugs. The post-treatment coproculture showed Haemonchus contortus and Strongyloides spp. larvae. Thus, the present study revealed presence of multiple anthelmintic resistance against fenbendazole, morantel and ivermectin in sheep of Mahendergarh district in Haryana.
How to cite this article:
Priyanka, Sukhdeep Vohra, Satyavir Singh and Arun K Sangwan. . The Pharma Innovation Journal. 2019; 8(8): 174-177.