Vol. 8, Issue 6 (2019)
Detection of cysteine proteinase gene hmcp5 of Haemonchus contortus by reverse transcription-polymerase chain reaction
S Sivajothi, VC Rayulu, T Prasada Rao, L Jeyabal, T Venkata Sai Kumar and KV Subramanyam
Barber’s pole worm cysteine proteinases are considered as the important candidates in the immunological control of haemonchosis in sheep and goats. The control of Haemonchus contortus is largely based on pasture management and the antihelmintics utilization. Due to indiscriminating use of drugs, occurrence of resistant to anthelmintics and it is need for the development of alternative control methods i.e. vaccine development. Cysteine proteases are considered as the excellent vaccine candidates due to their role in maintenance of worm nutrition as well as immune evasion. Cysteine proteases have been implicated for development of the vaccination because of their affinity purified detergent soluble extracts of Haemonchus contortus. The aim of the present study was to standardize the reverse transcription-polymerase chain reaction of Cysteine Proteinase gene hmcp5 of Haemonchus contortus. The characterization of cysteine protease genes in South Indian isolates may give ideas for control of Haemonchus contortus in future. It is essential for the further research protocols in development of the diagnostic or vaccination modalities.
How to cite this article:
S Sivajothi, VC Rayulu, T Prasada Rao, L Jeyabal, T Venkata Sai Kumar and KV Subramanyam. Detection of cysteine proteinase gene hmcp5 of <em>Haemonchus contortus </em>by reverse transcription-polymerase chain reaction. 2019; 8(6): 24-27.