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Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 7, Issue 6 (2018)
β - Galactosidase assay on microfluidic paper-based analytical devices (µPADs)
Author(s):
Anushree Lokur
Anushree Lokur
Abstract:
Enzyme assays using Microfluidic Paper-Based Analytical Devices (µPADs) are effective yet economical alternatives to conventional tube based enzyme assays. The current work involves standardisation of β-Galactosidase (β-gal) assay on µPADs using Chlorophenol red-β-D-galactopyranoside (CPRG) as a substrate. The assay was standardised with respect to substrate concentration, time of incubation, working range of enzyme and the limit of detection on the device. Presence of β-gal producers (coliforms) in spiked food samples using this assay was demonstrated as a proof of concept.
Enzyme assays using Microfluidic Paper-Based Analytical Devices (µPADs) are effective yet economical alternatives to conventional tube based enzyme assays. The current work involves standardisation of β-Galactosidase (β-gal) assay on µPADs using Chlorophenol red-β-D-galactopyranoside (CPRG) as a substrate. The assay was standardised with respect to substrate concentration, time of incubation, working range of enzyme and the limit of detection on the device. Presence of β-gal producers (coliforms) in spiked food samples using this assay was demonstrated as a proof of concept.
Pages: 103-106 | 1032 Views 142 Downloads
How to cite this article:
Anushree Lokur. β - Galactosidase assay on microfluidic paper-based analytical devices (µPADs). Pharma Innovation 2018;7(6):103-106.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
