Vol. 7, Issue 4 (2018)

Author(s):
Kontham Sampath, Vishweshwar Kumar Ganji and Pottabathula Mallesh

Abstract:
The genomic DNA extraction and PCR amplification of gene fragment from whole blood sample is a cumbersome process. Here in this study we described a direct PCR amplification of gene fragment from the whole blood sample of sheep bypassing the genomic DNA extraction steps. The detailed technique is explained and the results are suggestive of high accuracy in gene amplification. This can be used to cut short the time and is economic for gene amplification from whole blood sample with the same available reagents as for the conventional method.