Vol. 7, Issue 2 (2018)

Author(s):
Ankita Sinha, Harshita, Dr. Ramesh Singh, Saurav Govind Rao and Ankur Verma

Abstract:
Five different culture media namely, Potato dextrose agar, Potato carrot agar, Carrot agar, Czapex dox agar and Vegetable juice, were evaluated for enhanced growth performance of Trichoderma harzianum as well as Trichoderma viride. For both species, the highest radial growth were found in Potato dextrose agar (PDA) as 90.0 mm and lowest in Vegetable juice (V₈) as 50.0 mm and 65.5 mm for T. harzianum and T. viride respectively. Then Trichoderma spp. was grown on PDA at various temperature i.e. at 15 °C, 25 °C, 30 °C, 35 °C and also at various pH levels viz; 5, 6, 7, 8 and 9. For both Trichoderma spp. the highest radial growth was obtained at 25 °C and lowest at 15 °C and 45 °C while mycelial weight was highest at pH 6 as 1.13g and at pH 5 as 0.63g for T. harzianum and T. viride respectively.