Vol. 7, Issue 11 (2018)

Author(s):
Dhivahar M, Devigasri C, Surya Sankar, Niranjana S Rajalakshmi, Nair Aswathy, Mini M and Anu Bosewell

Abstract:
A six month -old pigeon with clinical signs of diarrhoea and torticollis was presented to the Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy for disease investigation. The bird was sacrificed and samples from lungs, trachea, air sacs, liver, spleen, heart blood, intestine and brain were collected aseptically for isolation of bacteria and fungi. Nucleic acid was extracted from the tissue samples collected and were subjected to polymerase chain reaction (PCR) assays for the detection of Newcastle disease virus, Infectious Bronchitis virus, Infectious Laryngotracheitis virus and Mycoplasma gallisepticum. No organism of pathogenic significance could be isolated by bacterial and fungal culture. On PCR, amplicons could be detected at 236 bp and 386 bp, respectively for Newcastle disease and Infectious bronchitis.