Vol. 7, Issue 11 (2018)

Author(s):
J Shyam Sundar, R Gloria Jemmi Christobel, N Kasi Selvi, MP Abirami and Dr. Shila Samuel

Abstract:
The present study reports the successful synthesis of silver nanoparticles (Ag NPs) by plant-mediated green route and antimicrobial and anticancer activity investigated. The seed extract of locally sourced Caesalpinia bonducella acted as the reducing agents/capping agents. The CB seed AgNPs characteristics elucidated under UV–visible spectroscopy, Fourier transform infrared spectroscopy and transmission electron microscopy. The antimicrobial activity of the synthesized nanoparticles was tested against various pathogenic bacteria. Anticancer effect in Human breast cancer cell line MCF-7 was investigated. The synthesized AgNPs exhibited a maximum absorption at 455 nm and the Fourier transform infrared spectroscopy analysis confirmed the conversion of Ag+ ions to AgNPs due to the reduction by capping materials such as flavonoids and alkaloids of seed extract. The HR-TEM analysis revealed that they are spherical ranging 40nm. Significant growth inhibitions were found using analysis of variance (ANOVA), SPSS statistical tool at P<0.05. The highest activity of CB seed Ag NPs was against P. aeruginosa. Cytotoxicity of biosynthesized AgNPs against in vitro Human breast cancer cell line MCF-7 showed a dose-response activity with an IC50 value of 75μg/ml. Hence, the findings of this research suggest potential applications of the bionanoparticles as a candidate for therapeutic drugs because of their biogenic nature.