Vol. 7, Issue 11 (2018)

Author(s):
Kunzes Dolma, Jyoti Nagar, Deb Endra Kumar Sahoo and Ashish

Abstract:
Is hydrophobic collapse model (HCM) is applicable to multi-domain proteins resulting from gene duplication/triplication? To probe this, we analyzed small angle X-ray scattering (SAXS) datasets on six-domain protein, gelsolin as function of increasing and decreasing urea. Kratky and Porod exponent plots and radius of gyration (R_G) values indicated that with increase in urea, gelsolin transformed from globular to Gaussian-chain like to unfolded state. Upon reversal by dialysis at 4°C, profiles reversed back, except in presence of Ca²⁺ ions or low pH or at increased temperature. Overall, our results uphold that HCM is extendable to gelsolin if done under native conditions.