Vol. 6, Issue 4 (2017)

Author(s):
Ramsinh L, Ramchandra Reddy K, Aruna Kumari G, Gopala Reddy A, Sarjan Rao K and Ashok Kumar D

Abstract:
The aim of the study was to determine concentration of the Estrus sheep serum (ESS) for sheep in vitro oocyte maturation. Sheep ovaries were collected from a local abattoir and transported within 1 h to the laboratory in a warm saline solution (30 – 35 ^oC), Cumulus-oocyte complexes (COC’s) were obtained by aspirating of follicles, washed in TCM-199 modification with 50 g/ml gentamycin, The COC’s were randomly divided into Three groups. Treatment I (n = 100) COC’s were fresh and cultured in TCM-199 medium with 10% ESS + Estrdio 17β + 10 µg/ml FSH+10µg/ml LH. Treatment II (n = 100) COC’s were washed five times and cultured in TCM-199 medium supplemented with Estrdio 17β + 10 µg/ml FSH+10µg/ml LH. Treatment III (n = 100) COC’s were washed five times and cultured in TCM-199 medium and gentamycin treated as control. the evidence of different stages of maturation. Significantly higher (p<0.05) maturation rates of oocytes (95%, 61% and 10% were observed in Treatment I, IIand III However, significant difference was observed between the Treatment I,II and III.