Vol. 6, Issue 3 (2017)

Author(s):
Shaul Ahmed R, Punit R Bhatt, Chirag M Modi, Vipul B Chauhan, Kajal B Pandya, Urvesh D Patel and Harshad B Patel

Abstract:
Glycyrrhiza glabra and Tinospora cordifolia are traditional medicinal plants which possess anti-spasmodic, anti-inflammatory, anti-cancerous, and antioxidant properties. The present study was carried out to evaluate an in-vitro anti-diabetic activity of different extracts of G. glabra root and T. cordifolia stem in proportions of 1:2 and 2:1 by α-amylase inhibition method. Chloroform, methanol and water extracts of both plants were prepared by soxhlet apparatus. Various concentrations from 10 to 100 µg/mL were used to determine the activity. Acarbose was used as standard test substance. Water extracts in 1:2 and 2:1 ratio inhibits α-amylase significantly (p<0.05) with 53.69±2.14 and 52.89±1.40 percent, respectively. In case of methanolic extract, 2:1 ratio of both plants inhibits 53.95±0.66 percent (p<0.05), whereas 1:2 ratio of same extract by 48.12±1.40 percent. Chloroform extracts didn’t show any inhibition against α-amylase. Presence of triterpenoid in the methanol and water extracts of both plants might be responsible for antidiabetic activity.