Vol. 6, Issue 2 (2017)

Author(s):
Bichamma V, Gopala Reddy A, Vijay Kumar M, Usha Rani K, Anil Kumar B , Laxman M and Ramakoti Reddy M

Abstract:
An experimental study was conducted to evaluate the druggability prioritization targets of Salmonella gallinarum in commercial broilers in two experimental phases. In phase I of the experiment total proteome of pathogen Vs host comparison using comparative genomics was done followed by protein docking of the target proteins of the pathogen and drug target identification and then calculation of drug prioritization parameters for therapeutic target. Pharmacological evaluation of in vitro anti-biogram test of Salmonella gallinarum was done in phase II of experiment. 3965 proteins of S. gallinarum were compared with 24,068 proteins of Gallus gallus by BLASTP analysis. Out of 12 ligands, which were the lead molecules based on in silico virtual screening, used in vitro agar diffusion (for MIC), none of them was found effective as there was no inhibition. Further, minimum inhibitory concentration (MIC) was assessed with seven antibiotics (enrofloxacin, moxifloxacin, azithromycin, erythromycin, chloramphenicol, cefpodoxime and clindamycin), Salmonella gallinarum showed moderate efficacy in terms of zone of inhibition to one antibiotic (Cefpodoxime).