Vol. 6, Issue 12 (2017)
GC-MS analysis, Antibacterial, Antioxidant and Brine Shrimp Lethality Assay of Murraya koenigii Spreng
Ram Lal Shrestha
Extraction of essential oil of leaves of Murraya koenigii Spreng. was done by hydro distillation in Clevenger apparatus. Also, it was subjected to extraction with methanol. The composition of essential oil so collected were determined by GC-MS system and showed the presence of 23 different compounds. Caryophyllene (27.89%), α-Pinene (14.22%), Caryophyllene oxide (7.74%), α-Caryophyllene (7.25%), β-Pinene (6.01%), α-Selinene (5.36%) and D-Limonene (5.04%) were the most abundant. Antibacterial, antioxidant activity and brine shrimp lethality assay were studied. Oil exhibited moderate antibacterial activity. Extract showed efficient DPPH antioxidant activity with IC50 93.2 ± 7.5μg/mL. From Brine shrimp lethality assay, the LD50 of the sample of plant was found to be 125μg/mL.
How to cite this article:
Ram Lal Shrestha. GC-MS analysis, Antibacterial, Antioxidant and Brine Shrimp Lethality Assay of Murraya koenigii Spreng. 2017; 6(12): 35-38.