Abstract:With an objective to develop criteria for further standardization techniques of extracts, obtained from above-ground parts of promising hypoglycemic plant goat’s rue (
Galega officinalis L.), we have carried out analysis of contents of phenolic compounds and microelements.
The article describes the results of the research concerning qualitative and quantitative composition of phenolic compounds in ethanolic extracts of the goat’s rue (Galega officinalis L.) herb by the methods of high-performance liquid chromatography and spectrophotometry. The results of detection of phenolic compounds in aqueous-alcoholic extracts from aerial portion of goat’s rue showed the occurrence of 48 compounds, amongst which 7 compounds were identified, such as caffeic acid, ferulic acid, chicoric acid, rutin, quercetin, hyperoside, and apigenin. The optimal extractant for the maximum extraction of the flavonoids’ totality from goat’s rue (Galega officinalis L) herb has been determined and the fact that the highest yield of flavonoids (0.097%) was extracted with 50% alcoholic extract has been established.
The outcomes of this experimental study carried out by the method of atomic absorption spectrometry with electrothermal atomization, for the content of trace elements, which might have an impact for the herb’s hypoglycemic properties, were as follows: manganese – 23.8 µg/g, copper – 14.2 µg/g, selenium – 0.27 µg/g.