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Vol. 6, Issue 11 (2017)

Identification of goat poxvirus by agar gel immunodiffusion, counter immunoelectrophoresis and indirect enzyme-linked immunosorbent assay from an outbreak in Bidar

Author(s):
Dr. Abhilash B, Basavaraj A and Shrikant MB
Abstract:
Fourteen serum samples from osmanabadi goat breeds suspected for goat pox cases in Islampur village of Humnabad taluk, Bidar district were investigated by three different serological assays: agar gel immunodiffusion (AGID), counter immunoelectrophoresis (CIE) and indirect enzyme-linked immunosorbent assay (i-ELISA). All the sera samples collected were positive by i-ELISA and 9 were positive by CIE and 6 by AGID. The i-ELISA sensitivity was 100%, while that of CIE and AGID was 64% and 42%, respectively. The i-ELISA described combines high specificity and sensitivity in detection of goat poxvirus. The suspected samples were positive for goat pox disease and an i-ELISA was therefore shown to be the method of choice for goat poxvirus diagnosis directly from serum samples during an outbreak.
Pages: 49-50  |  360 Views  8 Downloads
How to cite this article:
Dr. Abhilash B, Basavaraj A and Shrikant MB. Identification of goat poxvirus by agar gel immunodiffusion, counter immunoelectrophoresis and indirect enzyme-linked immunosorbent assay from an outbreak in Bidar. 2017; 6(11): 49-50.
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