Vol. 6, Issue 1 (2017)

Author(s):
Shah Md. Wasif Faisal, Amer Khorshed Alam, Md. Nilavrow Sajed and Nur-E-Hasnat

Abstract:
The purpose of this study was to find out the prevalence and antibiotic sensitivity pattern of Salmonella typhi and Salmonella paratyphi isolated from blood samples of typhoid suspected patients of both sexes in Dhaka city, Samples were inoculated on MacConkey agar media and Blood agar media for primary identification of Salmonella species. Depending on colony formation, pigmentation, elevation and margins, colonies were presumably identified. Then the presumed isolates were further tested for more confirmation. All isolates were examined through biochemical tests and susceptibility test was performed by using different antimicrobial agents. Out of total 350 samples 123(35.14%) were positive for Salmonella typhi and Salmonella paratyphi, among the positive samples 112 (91%) were Salmonella typhi and 11 (9%) were Salmonella paratyphi. The growth positive rate in the samples of the two genders for Salmonella typhi- Male: 64% and Female: 36%, and for Salmonella paratyphi male- 63.6% and female- 36.4%. The samples of Salmonella typhi were most sensitive to Ceftriaxone, Ciprofloxacin and Gentamycin followed by, Cefixime, Cotrimoxazole, Chloramphenicol and Ampicillin and was least sensitive to Nalidixic acid followed by Azithromycin. Salmonella paratyphi was found to be most sensitive to Cefixime, Ceftriaxone, Ciprofloxacin and Gentamycin followed by Chloramphenicol, Cotrimoxazole and Ampicillin and was least sensitive to both Nalidixic acid and Azithromycin. This study showed that in the city of Dhaka males are more likely to be affected by typhoid fever than females. This study also showed the percentages of patients being affected by Salmonella typhi are more than Salmonella paratyphi.