Vol. 6, Issue 1 (2017)
Qualitative and quantitative estimation of phytosterols in Achyranthes aspera and Cissus quadrangularis by HPLC
Tamanna Talreja, Mangesh Kumar, Asha Goswami, Ghanshyam Gahlot, Surendra Kumar Jinger and Tribhuwan Sharma
Reversed phase high performance liquid chromatography (RP-HPLC) with UV detector was done for the analysis of four naturally occurring phytosterols of Achyranthes aspera and Cissus quadrangularis. The separation of filtered plant extracts as well as a mixture of authentic standard phytosterols samples of Campesterol, Stigmasterol, β-Sitosterol, Stigmastanol was done within 25 min. Running conditions included: injection volume 10µl; mobile phase: acetonitrile: water (50:50) containing 3% water, flow rate 1 ml/min; and detection at 205nm. Identifications of specific phytosterols are made by comparing their retention times with those of the standards. Three phytosterols in Achyranthes aspera namely Campesterol, Stigmasterol, β-Sitosterol and two phytosterols namely Campesterol, Stigmasterol in Cissus quadrangularis were detected by HPLC.
How to cite this article:
Tamanna Talreja, Mangesh Kumar, Asha Goswami, Ghanshyam Gahlot, Surendra Kumar Jinger and Tribhuwan Sharma. . The Pharma Innovation Journal. 2017; 6(1): 76-79.